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我正在尝试运行下面的脚本。但我得到了错误：

------------- 异常 ------------- 味精：找不到 clustalw 的可执行文件。path="clustalw.exe" STACK Bio::Tools::Run::WrapperBase::executable C:/Perl64/site/lib/Bio/Tools/Run/ WrapperBase.pm:340 STACK Bio::Tools::Run: :Alignment::Clustalw::_run C:/Perl64/site/lib/Bio/Tools/Run/Alignment/Clustalw.pm:752 堆栈 Bio::Tools::Run::Alignment::Clustalw::align C: /Perl64/site/lib/Bio/Tools/R un/Alignment/Clustalw.pm:515 堆栈顶层 a.pl:29

如何让 perl 找到我的clustalw.exe？

我认为已经通过在开始时设置环境变量来做到这一点$ENV{CLUSTALDIR}。

#!/usr/bin/perl
$ENV{CLUSTALDIR} = 'C:\Program Files (x86)\ClustalW2';
use warnings;
use strict;

use Bio::AlignIO;
use Bio::SeqIO;
use Bio::Tools::Run::Alignment::Clustalw;

my $file = <>; # Get file name from command prompt.
my $factory = Bio::Tools::Run::Alignment::Clustalw->new(-matrix => 'BLOSUM');
my $ktuple = 3;
$factory->ktuple($ktuple);

my $inseq = Bio::SeqIO->new(
                    -file => "<$file",
                    -format => "fasta"
                   );

my $seq;
my @seq_array;
while ($seq = $inseq->next_seq) {
    push(@seq_array, $seq);
}

# Now we do the actual alignment.
my $seq_array_ref = \@seq_array;
my $aln = $factory->align($seq_array_ref);
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