r - 使用 DEXSeqDataSetFromHTSeq 时出错

Question

目前我正在尝试了解DEXSeq包。我有一个设计 tsv 文件和 7 个包含计数的文件。现在想运行以下命令

library("DEXSeq");

design=read.table("dexseq_design.tsv", header=TRUE, row.names=1);

ecs = DEXSeqDataSetFromHTSeq(countfiles=c("M0.txt", "M1.txt", "M2.txt", "M3.txt", "M4.txt", "M5.txt", "M6.txt", "M7.txt"), design=design, flattenedfile="genome.chr.gff");

最后一个命令给出和错误

Error in class(sampleData) %in% c("data.frame") : 
error in evaluating the argument 'x' in selecting a method for function '%in%':
Error: argument "sampleData" is missing, with no default

此错误是什么意思以及如何解决？加载包DEXSeq时出现警告

Warning message:
  replacing previous import by ‘ggplot2::Position’ when loading ‘DESeq2’