0

我在 RNAseq 数据上计算 LIMMA。当我使用这个公式时:

~feature + age  + (1 | sex) + (1 | group) + (1 | id)

里面voomWithDreamWeights()我没有问题。当我向此公式添加变量时,出现以下错误:

Error in .fitVarPartModel(exprObj, formula, data, REML = REML, useWeights = useWeights,  : 
  the fixed-effects model matrix is column rank deficient (rank(X) = 339 < 340 = p);
the fixed effects will be jointly unidentifiable 

Suggestion: rescale fixed effect variables.
This will not change the variance fractions or p-values

年龄和给我这个问题的变量是连续变量。

4

0 回答 0