我在 RNAseq 数据上计算 LIMMA。当我使用这个公式时:
~feature + age + (1 | sex) + (1 | group) + (1 | id)
里面voomWithDreamWeights()
我没有问题。当我向此公式添加变量时,出现以下错误:
Error in .fitVarPartModel(exprObj, formula, data, REML = REML, useWeights = useWeights, :
the fixed-effects model matrix is column rank deficient (rank(X) = 339 < 340 = p);
the fixed effects will be jointly unidentifiable
Suggestion: rescale fixed effect variables.
This will not change the variance fractions or p-values
年龄和给我这个问题的变量是连续变量。