我是一个相当新的 Circos 用户,我试图让 Circos 不显示任何低于一定长度的链接,比如说 10000。这是我当前的链接部分,包括规则:
<links>
z = 5
radius = 0.65r
bezier_radius = 0.4r
bezier_radius_purity = 0.45
crest = 0.4
perturb = yes
<link segdup>
show = yes
color = black_a8
thickness = 2
record_limit=5000
file = minDist_10K_KO.circos.interactions.txt
<rules>
<rule>
importance = 205
condition = _THICKNESS1_ > 20
thickness = 20
</rule>
<rule>
importance = 185
condition = abs(var(pos1) - var(pos2)) < 100000
show = no
</rule>
<rule>
importance = 200
condition = _THICKNESS1_ < -20
thickness = 20
</rule>
<rule>
importance = 195
condition = _THICKNESS1_ < 0
thickness = eval(abs(_THICKNESS1_))
</rule>
<rule>
importance = 190
condition = _THICKNESS1_ < 1
thickness = 1
</rule>
</rules>
</link>
</links>
应该改变事物的规则是第二条规则,即:
<rule>
importance = 185
condition = abs(var(pos1) - var(pos2)) < 10000
show = no
</rule>
但是,无论我将值更改为什么,图像中显示的链接都不会改变。此外,现在线条非常细——不太清楚为什么还会发生这种情况。我试过改变规则的重要性,但这也不起作用。最后,这是我的交互文件示例:
interaction1 chr10 117930136 117933136 thickness=12
interaction1 chr10 117917971 117920971 thickness=12
interaction2 chr10 117513537 117516537 thickness=12
interaction2 chr10 117503002 117506002 thickness=12
interaction3 chr10 118091884 118094884 thickness=10
interaction3 chr10 118074929 118077929 thickness=10
interaction4 chr10 118063858 118066858 thickness=10
interaction4 chr10 118054121 118057121 thickness=10
interaction5 chr10 117972111 117975111 thickness=10
interaction5 chr10 117961998 117964998 thickness=10
interaction6 chr10 117527213 117530213 thickness=10
interaction6 chr10 117501960 117504960 thickness=10
interaction7 chr10 117530894 117533894 thickness=10
interaction7 chr10 117501760 117504760 thickness=10
interaction8 chr10 117518745 117521745 thickness=10
interaction8 chr10 117501988 117504988 thickness=10
interaction9 chr10 117527102 117530102 thickness=8
interaction9 chr10 117513846 117516846 thickness=8
interaction10 chr10 118000811 118003811 thickness=8
interaction10 chr10 117990699 117993699 thickness=8
interaction11 chr10 117584531 117587531 thickness=8
interaction11 chr10 117570250 117573250 thickness=8
interaction12 chr10 117539379 117542379 thickness=8
interaction12 chr10 117527172 117530172 thickness=8
有人知道发生了什么吗?