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我是一个相当新的 Circos 用户,我试图让 Circos 不显示任何低于一定长度的链接,比如说 10000。这是我当前的链接部分,包括规则:

<links>

    z = 5

    radius = 0.65r
    bezier_radius = 0.4r
    bezier_radius_purity = 0.45
    crest = 0.4
    perturb = yes

    <link segdup>
        show = yes
        color = black_a8
        thickness = 2
        record_limit=5000
        file = minDist_10K_KO.circos.interactions.txt
                    <rules>
                        <rule>
                                importance = 205
                                condition = _THICKNESS1_ > 20
                                thickness = 20
                        </rule>
                        <rule>
                                importance = 185
                                condition = abs(var(pos1) - var(pos2)) < 100000
                                show = no
                        </rule>   
                        <rule>
                                importance = 200
                                condition = _THICKNESS1_ < -20
                                thickness = 20
                        </rule>
                        <rule>
                                importance = 195
                                condition = _THICKNESS1_ < 0
                                thickness = eval(abs(_THICKNESS1_))
                        </rule>
                        <rule>
                                importance = 190
                                condition = _THICKNESS1_ < 1
                                thickness = 1
                        </rule>
                </rules>
    </link>
</links>

应该改变事物的规则是第二条规则,即:

                    <rule>
                            importance = 185
                            condition = abs(var(pos1) - var(pos2)) < 10000
                            show = no
                    </rule> 

但是,无论我将值更改为什么,图像中显示的链接都不会改变。此外,现在线条非常细——不太清楚为什么还会发生这种情况。我试过改变规则的重要性,但这也不起作用。最后,这是我的交互文件示例:

interaction1 chr10 117930136 117933136 thickness=12
interaction1 chr10 117917971 117920971 thickness=12
interaction2 chr10 117513537 117516537 thickness=12
interaction2 chr10 117503002 117506002 thickness=12
interaction3 chr10 118091884 118094884 thickness=10
interaction3 chr10 118074929 118077929 thickness=10
interaction4 chr10 118063858 118066858 thickness=10
interaction4 chr10 118054121 118057121 thickness=10
interaction5 chr10 117972111 117975111 thickness=10
interaction5 chr10 117961998 117964998 thickness=10
interaction6 chr10 117527213 117530213 thickness=10
interaction6 chr10 117501960 117504960 thickness=10
interaction7 chr10 117530894 117533894 thickness=10
interaction7 chr10 117501760 117504760 thickness=10
interaction8 chr10 117518745 117521745 thickness=10
interaction8 chr10 117501988 117504988 thickness=10
interaction9 chr10 117527102 117530102 thickness=8
interaction9 chr10 117513846 117516846 thickness=8
interaction10 chr10 118000811 118003811 thickness=8
interaction10 chr10 117990699 117993699 thickness=8
interaction11 chr10 117584531 117587531 thickness=8
interaction11 chr10 117570250 117573250 thickness=8
interaction12 chr10 117539379 117542379 thickness=8
interaction12 chr10 117527172 117530172 thickness=8

有人知道发生了什么吗?

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