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我正在尝试使用 Monocle（不是 Monocle3）将 Seurat3 对象中的数据集作为轨迹的子集，如下所示：

mono <- subset(Seurat.combined,idents=c(0,2,3,5))

然后使用newCellDataSet构造单片眼镜CDS(mono_cds)，调用reduceDimension时出现错误：

mono_cds <- reduceDimension(mono_cds, max_components = 2,
                        reduction_method = "DDRTree")

DDRTree will be eventually deprecated in reduceDimension call and be used in learnGraph function instead. We are calling learnGraph for you now.
Error in RANN::nn2(data, data, k + 1, searchtype = "standard") : 
  no points in data!
In addition: Warning message:
In (function (data, pd, k = 20, weight = F, louvain_iter = 1, resolution = NULL,  :RANN counts the point itself, k must be smaller than
the total number of points - 1 (all other points) - 1 (itself)!
Timing stopped at: 0.008 0 0.008

问题可能是Seurat对象的子集引起的，如果我不子集数据集并使用整个Seurat3对象构建单片CDS，没有问题。

我正在使用 monocle_2.99.3 和 Seurat 3.1.2。

有什么帮助吗？谢谢！

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