2

我是 python 新手并运行命令:

点安装 pysam

结果是:

Collecting pysam
  Using cached https://files.pythonhosted.org/packages/25/7e/098753acbdac54ace0c6dc1f8a74b54c8028ab73fb027f6a4215487d1fea/pysam-0.15.4.tar.gz
    ERROR: Command errored out with exit status 1:
     command: 'c:\path\programs\python\python38\python.exe' -c 'import sys, setuptools, tokenize; sys.argv[0] = '"'"'C:\\path\\Local\\Temp\\pip-install-qzuue1yz\\pysam\\setup.py'"'"'; __file__='"'"'C:\\path\\Temp\\pip-install-qzuue1yz\\pysam\\setup.py'"'"';f=getattr(tokenize, '"'"'open'"'"', open)(__file__);code=f.read().replace('"'"'\r\n'"'"', '"'"'\n'"'"');f.close();exec(compile(code, __file__, '"'"'exec'"'"'))' egg_info --egg-base pip-egg-info
    Complete output (23 lines):
    # pysam: cython is available - using cythonize if necessary
    # pysam: htslib mode is shared
    # pysam: HTSLIB_CONFIGURE_OPTIONS=None
    '.' is not recognized as an internal or external command,
    operable program or batch file.
    '.' is not recognized as an internal or external command,
    operable program or batch file.
      File "<string>", line 1, in <module>
      File "C:\path\Local\Temp\pip-install-qzuue1yz\pysam\setup.py", line 241, in <module>
        htslib_make_options = run_make_print_config()
      File "C:\path\\Local\Temp\pip-install-qzuue1yz\pysam\setup.py", line 68, in run_make_print_config
        stdout = subprocess.check_output(["make", "-s", "print-config"])
      File "c:\path\programs\python\python38\lib\subprocess.py", line 411, in check_output
        return run(*popenargs, stdout=PIPE, timeout=timeout, check=True,
      File "c:\path\programs\python\python38\lib\subprocess.py", line 489, in run
      File "c:\path\programs\python\python38\lib\subprocess.py", line 854, in __init__
        self._execute_child(args, executable, preexec_fn, close_fds,
      File "c:\path\programs\python\python38\lib\subprocess.py", line 1307, in _execute_child
        hp, ht, pid, tid = _winapi.CreateProcess(executable, args,
    FileNotFoundError: [WinError 2] The system cannot find the file specified
    # pysam: htslib configure options: None
    ----------------------------------------
ERROR: Command errored out with exit status 1: python setup.py egg_info Check the logs for full command output.

这里有什么问题?

最初我收到关于未安装 cython 的错误,所以我运行了 pip install cython 并且能够毫无问题地运行它。

4

2 回答 2

3

PyPI有许多二进制轮子,但仅适用于 Linux 和 MacOS X。bioconda 的包也仅针对 Linux 和 OS X 编译。

当您尝试在 Windows 上安装 pysam 时,pip下载源代码分发pysam-0.15.4.tar.gz,解压缩并运行setup.y

pysam通过运行脚本来setup.py 配置库。这是一个 shell 脚本,它不能在没有 Unix 仿真层的 Windows 中运行。因此错误。htslibhtslib/configure

底线:就像许多与遗传学相关的软件(我有一些用 Python 和 Java 编写的软件的经验)pysam似乎只能在 Unix 上使用,最好是 Linux 或 OS X。

于 2020-02-13T01:54:23.320 回答
2

如果你有蟒蛇,试试这个:

conda install -c bioconda pysam

于 2020-02-12T22:37:05.550 回答