我正在尝试将 SNP 绘制到基因(或以下)上。我的代码如下:
#!/usr/bin/perl
use strict;
use warnings;
use Bio::Graphics;
use Bio::SeqFeature::Generic;
my @SNPs = "408777 408900 409100 409480";
my $gene_name = "GSTd10";
my $scaffold = "KB668289";
my $gene_start = 408763;
my $gene_end = 409489;
my $length = $gene_end - $gene_start + 50;
open my $png, ">", "$gene_name.png" or die "Cannot open $gene_name.png: $!\n";
#Create a panel for the image#
my $panel=Bio::Graphics::Panel->new(-offset => $gene_start, -length => $length, -width => 1000, -pad_left => 100, -pad_right => 10, -pad_top => 10);
my $track_whole=$panel->add_track(-glyph => 'graded_segments', -label => 1, -bgcolor => 'black', -font2color => 'black',);
my $feature= Bio::SeqFeature::Generic->new(-display_name => $gene_name, -start => $gene_start, -end => $gene_end,);
$track_whole->add_feature($feature);
my $track=$panel->add_track(-glyph => 'graded_segments', -label => 1, -bgcolor =>'blue', -min_score => 0, -max_score => 30, -font2color => 'black');
foreach my $SNP (@SNPs)
{
my $feature= Bio::SeqFeature::Generic->new(-label => $SNP, -start => $SNP, -end => $SNP);
$track->add_feature($feature);
}
#This will print out the final panel i.e. you must have created an object called $panel above
print $png $panel -> png;
每当我运行这个脚本时,我只会打印一行。
打印所有值的错误在哪里@SNPs
?另外,有没有办法打印^
而不是块?