r - 构建循环系统发育树

Question

我有一张与它们相关的基因和疾病表。我想构建一个系统发育树并将基因分组到它们的疾病中。下面是一个示例数据集，其中gene1 列属于 disease1，gene2 属于 disease2。主要是 gene1 和基因2彼此相关，并映射到它们所属的疾病。

gene1   gene2   disease1           disease2
AGTR1   ACHE    cancer              tumor
AGTR1   ACHE    parkinson's         asthma
ALOX5   ADRB1   myocardial infarct  heart failure
AR      ADORA1  breast cancer       anxiety disorder

为了我的目的，我想要一个循环的系统发育树，在下面的链接中给出：http: //itol.embl.de/itol.cgi

在 R 或任何软件中执行此操作的任何建议？

谢谢

我正在运行的代码，现在：

d=read.csv("genes_disease.txt",sep="\t",header=TRUE)
phyl_gad <-as.phylo(hclust(dist(d)))
 plot(phyl_gad,type="fan",edge.col=c("red","green","blue","orange","yellow","pink","magenta","white"),show.tip.label=FALSE)

如果我确实显示.tip.label = TRUE，则绘制的标签太多，使提示混乱。

我修改后的数据集现在只有两列，一列基因，一列疾病。

Answer 1

啊，我以前做过。正如布莱恩所说，你想使用这个ape包。假设你有一个hclust对象。例如，

library(ape)
fit<-hclust(d,method='ward')
plot(as.phylo(fit),type='fan',label.offset=0.1,no.margin=TRUE)

如果要修改树末端的颜色，可以使用cutree和tip.color参数。这将为不同的集群创建一组重复的颜色（例如，color=c('red','blue')在分支的末端会有交替的蓝色和红色文本。

nclus=...#insert number of clusters you want to cut to
color=...#insert a vector of colors here
fit<-hclust(d,method='ward')
color_list=rep(color,nclus/length(color))
clus=cutree(fit,nclus)
plot(as.phylo(fit),type='fan',tip.color=color_list[clus],label.offset=0.1,no.margin=TRUE) 

我不确定您要使用哪种类型的聚类方法（我使用的是 Ward 方法），但您就是这样做的。

Answer 2

我认为你想做的不是系统发育，而是距离聚类。这是一个可重复的例子。

library(XML)
library(RCurl)#geturl
library(rlist)
library(plyr)
library(reshape2)
library(ggtree)

#get the genes/ diseases info from internet
#example from http://www.musclegenetable.fr/
urllist<-paste0("http://195.83.227.65/4DACTION/GS/",LETTERS[1:24] )
theurl <- lapply(urllist, function(x) RCurl::getURL(x,.opts = list(ssl.verifypeer = T) ) )# wait
theurl2<-lapply(theurl, function(x) gsub("<span class='Style18'>","__",x))
tables <- lapply(theurl2, function (x) XML::readHTMLTable(x) )
tables2 <- lapply(tables, function(x) rlist::list.clean(x, fun = is.null, recursive = FALSE) )
unlist1 = lapply(tables2, plyr::ldply)
newdf<-do.call(rbind, unlist1)
colnames(newdf)[4]<-"diseases"
colnames(newdf)[2]<-"Gene"
newdf$gene<-sub("([A-z0-9]+)(__)(.*)","\\1",newdf$Gene)
newdf$diseases<-sub("(\\* )","",newdf$diseases, perl=T)

#split info of several diseases per gene, and simplify text 
#to allow better clustering
newdf2<-as.data.frame(data.table::setDT(newdf)[, strsplit(as.character(diseases), "* ", fixed=TRUE), by = .(gene, diseases)
][,.(diseases = V1, gene)])
newdf2$disease<-sub("([A-z0-9,\\-\\(\\)\\/ ]+)( \\- )(.*)","\\1",newdf2$diseases)
newdf2$disease<-gsub("[0-9,]","",newdf2$disease)
newdf2$disease<-gsub("( [A-Z]{1,2})$","",newdf2$disease)
newdf2$disease<-gsub("(\\-)","",newdf2$disease)
newdf2$disease<-gsub("\\s*\\([^\\)]+\\)","",newdf2$disease)
newdf2$disease<-gsub("\\s*type.*","",newdf2$disease, ignore.case = T)
newdf2$disease<-gsub("(X{0,3})(IX|IV|V?I{0,3})","", newdf2$disease)
newdf2$disease<-gsub("( [A-z]{1,2})$","",newdf2$disease)
newdf2$disease<-sub("^([a-z])(.*)","\\U\\1\\E\\2",newdf2$disease, perl=T)
newdf2$disease<-trimws(newdf2$disease)
newdf2<-newdf2[,c(2,3)]

#make clustering and tree
newcasted <- reshape2::dcast(newdf2, gene ~ disease)
phyl_gad <-ape::as.phylo(hclust(dist(newcasted)))

#use names of genes and diseases in tree
DT <- data.table::as.data.table(newdf2)
newdf4<-as.data.frame(DT[, lapply(.SD, paste, collapse=","), by = gene, .SDcols = 2])
newdf4$genemerge<-paste(newdf4$gene, newdf4$disease)
phyl_gad$tip.label<-newdf4$genemerge

#plot tree
ggtree::ggtree(phyl_gad, layout = "circular")+ ggtree::geom_tiplab2(offset=0.1, align = F, size=4)