'create a dtb for demo
Dim dtbSource As New DataTable("MyDataTable")
dtbSource.Columns.Add("Column1", GetType(String))
dtbSource.Columns.Add("Column2", GetType(String))
dtbSource.Columns.Add("Column3", GetType(String))
dtbSource.Rows.Add("1", "2", "3")
dtbSource.Rows.Add("2", "2", "3")
dtbSource.Rows.Add("3", "2", "3")
dtbSource.Rows.Add("4", "2", "3")
dtbSource.Rows.Add("5", "2", "3")
dtbSource.Rows.Add("6", "2", "3")
dtbSource.Rows.Add("7", "2", "3")
dtbSource.Rows.Add("8", "2", "3")
dtbSource.Rows.Add("9", "2", "3")
dtbSource.Rows.Add("10", "2", "3")
dtbSource.Rows.Add("11", "2", "3")
dtbSource.Rows.Add("12", "2", "3")
dtbSource.Rows.Add("13", "2", "3")
'now split the datatable
Dim n As Integer = 5 'number of rows per datatable
Dim dst As New DataSet("Output")
For i As Integer = 0 To dtbSource.Rows.Count - 1 Step n
  Dim intLastRow As Integer = i + n - 1
  If intLastRow > dtbSource.Rows.Count - 1 Then intLastRow = dtbSource.Rows.Count - 1
  Dim dtbNew As DataTable = dtbSource.Clone 'copy structure of original datatable 
  dtbNew.TableName = dtbSource.TableName & "_" & (i \ n).ToString
  For j As Integer = i To intLastRow
    dtbNew.ImportRow(dtbSource.Rows(j))
  Next j
  dst.Tables.Add(dtbNew)
Next i
'the split datatables are available in dst.Tables
MsgBox(dst.Tables(1).Rows(2).Item(0).ToString) 'table 2, row 3, column 1