我对 RNAseq 分析非常陌生。我正在尝试使用 Galaxy 运行 HTSeq，因为我不熟悉编码以及如何使用 Python 执行此操作。
我收到以下错误：
Use of uninitialized value $input_file in concatenation (.) or string at /mnt/galaxyTools/shed_tools/toolshed.g2.bx.psu.edu/repos/fcaramia/edger/86292c2b0ba9/edger/htseq.pl line 57.
Usage: file [-bchikLlNnprsvz0] [--apple] [--mime-encoding] [--mime-type]
[-e testname] [-F separator] [-f namefile] [-m magicfiles] file ...
file -C [-m magicfiles]
file [--help]
Use of uninitialized value $input_file in concatenation (.) or string at /mnt/galaxyTools/shed_tools/toolshed.g2.bx.psu.edu/repos/fcaramia/edger/86292c2b0ba9/edger/htseq.pl line 62.
接下来是一长串发现错误的位置。例如：
Error occured when reading first line of sam file.
Error:
[Exception type: StopIteration, raised in count.py:81]
Use of uninitialized value $sample in hash element at /mnt/galaxyTools/shed_tools/toolshed.g2.bx.psu.edu/repos/fcaramia/edger/86292c2b0ba9/edger/htseq.pl line 67.
Use of uninitialized value $files[0] in string eq at /mnt/galaxyTools/shed_tools/toolshed.g2.bx.psu.edu/repos/fcaramia/edger/86292c2b0ba9/edger/htseq.pl line 78.