因此，我使用 yuk 和 gnovice 的解决方案创建了另一个解决方案。当我玩弄这个解决方案时，我意识到我真的希望能够将输出用作“子图”并能够任意更改字母的颜色。
由于 yuk 使用嵌入了字母的编程放置的轴对象，因此修改他的代码以绘制到任意轴对象会非常烦人（尽管并非不可能）。由于 gnovice 的解决方案是从预先创建的文件中读取字母，因此很难修改代码以针对任意颜色方案或字母选择运行。所以我的解决方案使用了yuk解决方案中的“字母生成”代码和gnovice解决方案中的“图像叠加”方法。
还有大量的参数解析和检查。以下是我的综合解决方案......我只是为了完整性而将其包括在内，我显然无法赢得自己的赏金。我将让社区决定奖励，并在时限结束时将赏金奖励给评分最高的人......如果出现平局，我会将其奖励给代表最低的人（他们可能更“需要”它）。
function [npos, handle] = SeqLogoFig(SEQ, varargin)
% SeqLogoFig
% A function which wraps around the bioinformatics SeqLogo command
% and creates a figure which is actually a MATLAB figure. All
% agruements for SEQLOGO are passed along to the seqlogo calculation.
% It also supports extra arguements for plotting.
%
% [npos, handle] = SeqLogoFig(SEQ);
%
% SEQ A multialigned set of sequences that is acceptable
% to SEQLOGO.
% npos The positions that were actually plotted
% handle An axis handle to the object that was plotted.
%
% Extra Arguements:
%
% 'CUTOFF' A bit-cutoff to use for deciding which columns to
% plot. Any columns that have a MAX value which is
% greater than CUTOFF will be provided. Defaults to
% 1.25 for NT and 2.25 for AA.
%
% 'TOP-N' Plots only the top N columns as ranked by thier MAX
% bit conservation.
%
% 'AXES_HANDLE' An axis handle to plot the seqlogo into.
%
% 'INDS' A set of indices to to plot. This overrides any
% CUTOFF or TOP-N that were provided
%
%
%
%
%% Parse the input arguements
ALPHA = 'nt';
MAX_BITS = 2.5;
RES = [200 80];
CUTOFF = [];
TOPN = [];
rm_inds = [];
colors = [];
handle = [];
npos = [];
for i = 1:2:length(varargin)
if strcmpi(varargin{i}, 'alphabet')
ALPHA = varargin{i+1};
elseif strcmpi(varargin{i}, 'cutoff')
CUTOFF = varargin{i+1};
%we need to remove these so seqlogo doesn't get confused
rm_inds = [rm_inds i, i+1]; %#ok<*AGROW>
elseif strcmpi(varargin{i}, 'colors')
colors = varargin{i+1};
rm_inds = [rm_inds i, i+1];
elseif strcmpi(varargin{i}, 'axes_handle')
handle = varargin{i+1};
rm_inds = [rm_inds i, i+1];
elseif strcmpi(varargin{i}, 'top-n')
TOPN = varargin{i+1};
rm_inds = [rm_inds i, i+1];
elseif strcmpi(varargin{i}, 'inds')
npos = varargin{i+1};
rm_inds = [rm_inds i, i+1];
end
end
if ~isempty(rm_inds)
varargin(rm_inds) = [];
end
if isempty(colors)
colors = GetColors(ALPHA);
end
if strcmpi(ALPHA, 'nt')
MAX_BITS = 2.5;
elseif strcmpi(ALPHA, 'aa')
MAX_BITS = 4.5;
end
if isempty(CUTOFF)
CUTOFF = 0.5*MAX_BITS;
end
%% Calculate the actual seqlogo.
wm = seqlogo(SEQ, varargin{:}, 'displaylogo', false);
%% Generate the letters
letters = wm{1};
letter_wins = cell(size(letters));
[~, loc] = ismember(letters, colors(:,1));
loc(loc == 0) = size(colors,1);
clr = cell2mat(colors(loc, 2)); % corresponding colors
for t = 1:numel(letters)
hf = figure('position',[200 200 100 110],'color','w');
ha = axes('parent',hf, 'visible','off','position',[0 0 1 1]);
ht = text(50,55,letters(t),'color',clr(t,:),'units','pixels',...
'fontsize',100,'fontweight','norm',...
'vertical','mid','horizontal','center');
F = getframe(hf); % rasterize the letter
img = F.cdata;
m = any(img < 255,3); % convert to binary image
m(any(m,2),any(m,1))=1; % mask to cut white borders
letter_wins{t} = reshape(img(repmat(m,[1 1 3])),[sum(any(m,2)) sum(any(m,1)) 3]);
close(hf);
end
%% Use the letters to generate a figure
%create a "image" that will hold the final data
wmat = wm{2};
if isempty(npos)
if isempty(TOPN)
npos = find(any(wmat>CUTOFF,1));
else
[~, i] = sort(max(wmat,[],1), 'descend');
npos = sort(i(1:TOPN));
end
end
fig_data = 255*ones(RES(1), RES(2)*(length(npos)+1)+length(npos)*2,3);
bitscores = linspace(0, MAX_BITS, size(fig_data,1));
tick_pos = zeros(length(npos),1);
% place images of letters
for i=1:length(npos)
[wms idx] = sort(wmat(:,npos(i)), 'descend'); % largest on the top
bits = [flipud(cumsum(flipud(wms))); 0];
let_data = letter_wins(idx(wms>0));
for s=1:length(let_data)
start_pos = find(bitscores>=bits(s),1);
end_pos = find(bitscores<=bits(s+1),1, 'last');
if isempty(start_pos) || isempty(end_pos) || end_pos > start_pos
continue
end
img_win = imresize(let_data{s}, [start_pos-end_pos, RES(2)]);
fig_data(start_pos-1:-1:end_pos, (i*RES(2)-RES(2)*.5:i*RES(2)+RES(2)*.5-1)+2*i,:) = img_win;
end
tick_pos(i) = i*RES(2)+2*i;
end
if ~isempty(handle)
image(handle,[0 size(fig_data,2)], [0 MAX_BITS],fig_data./255)
else
handle = image([0 size(fig_data,2)], [0 MAX_BITS],fig_data./255);
end
set(gca, 'ydir', 'normal', 'xtick', tick_pos, ...
'userdata', tick_pos, 'xticklabel', npos);
xlabel('position')
ylabel('bits')
function colors = GetColors(alpha)
% get the standard colors for the sequence logo
if strcmpi(alpha, 'nt')
colors = cell(6,2);
colors(1,:) = {'A', [0 1 0]};
colors(2,:) = {'C', [0 0 1]};
colors(3,:) = {'G', [1 1 0]};
colors(4,:) = {'T', [1 0 0]};
colors(5,:) = {'U', [1 0 0]};
colors(6,:) = {'', [1 0 1]};
elseif strcmpi(alpha, 'aa')
colors = cell(21,2);
colors(1,:) = {'G', [0 1 0]};
colors(2,:) = {'S', [0 1 0]};
colors(3,:) = {'T', [0 1 0]};
colors(4,:) = {'Y', [0 1 0]};
colors(5,:) = {'C', [0 1 0]};
colors(6,:) = {'Q', [0 1 0]};
colors(7,:) = {'N', [0 1 0]};
colors(8,:) = {'A', [1 165/255 0]};
colors(9,:) = {'V', [1 165/255 0]};
colors(10,:) = {'L', [1 165/255 0]};
colors(11,:) = {'I', [1 165/255 0]};
colors(12,:) = {'P', [1 165/255 0]};
colors(13,:) = {'W', [1 165/255 0]};
colors(14,:) = {'F', [1 165/255 0]};
colors(15,:) = {'M', [1 165/255 0]};
colors(16,:) = {'D', [1 0 0]};
colors(17,:) = {'E', [1 0 0]};
colors(18,:) = {'K', [0 0 1]};
colors(19,:) = {'R', [0 0 1]};
colors(20,:) = {'H', [0 0 1]};
colors(21,:) = {'', [210/255 180/255 140/255]};
else
error('SeqLogoFigure:BADALPHA', ...
'An unknown alphabet was provided: %s', alpha)
end
我已将此提交给 Mathworks FileExchange ... 批准后，我会发布一个链接。
我唯一烦恼的是，当它创建字母图像时，它会快速显示小图形窗口。如果有人知道可以避免的技巧，我很乐意听到。
编辑：Mathworks 已批准我提交的文件...您可以在此处的 FileExchange 下载它：http: //www.mathworks.com/matlabcentral/fileexchange/27124
