所以我想计算每一列的总和，结果会发生变化。我不知道如何处理它。
我的数据：
> head(df)
K 2 5 4 2
L 2 1 4 1
M 1 3 4 3
N 3 2 1 1
Sum 7 8 11 13
所以你看到结果不正确。第一列的总和在第二列，第一列是最后一列的总和。如何处理？
我使用该代码来计算总和：
df <- suppressWarnings(rbind(data, Sum=colSums(data[, -1])))
我的数据是这样的：
> dput(head(data,4))
structure(list(Name = structure(c(95L, 331L, 161L, 156L
), .Label = c(" 1-deoxy-D-xylulose 5-phosphate reductoisomerase ",
" 2-cysteine peroxiredoxin B ", " 2-oxoacid dehydrogenases acyltransferase family protein ",
" 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein ",
" 26S proteasome, regulatory subunit Rpn7;Proteasome component (PCI) domain ",
" 3-dehydroquinate synthase, putative ", " 3-ketoacyl-acyl carrier protein synthase I ",
" 3-ketoacyl-acyl carrier protein synthase III ", " 4-hydroxy-3-methylbut-2-enyl diphosphate reductase ",
" 40s ribosomal protein SA ", " 5'adenylylphosphosulfate reductase 2 ",
" AAA-type ATPase family protein ", " ACC oxidase 2 ", " acetoacetyl-CoA thiolase 2 ",
" ACT domain-containing small subunit of acetolactate synthase protein ",
" actin 7 ", " actin 8 ", " adenosine kinase 1 ", " adenosine kinase 2 ",
" adenylosuccinate synthase ", " ADP-glucose pyrophosphorylase family protein ",
" ADP glucose pyrophosphorylase 1 ", " ADP/ATP carrier 1 ",
" Aha1 domain-containing protein ", " alanine:glyoxylate aminotransferase ",
" alanine:glyoxylate aminotransferase 2 ", " Alba DNA/RNA-binding protein ",
" Aldolase-type TIM barrel family protein ", " Aldolase superfamily protein ",
" alkenal reductase ", " allene oxide synthase ", " Alpha-S1 casein precursor - Bos taurus (Bovine).",
" alpha/beta-Hydrolases superfamily protein ", " amidase 1 ",
" Amino acid dehydrogenase family protein ", " ankyrin repeat-containing 2B ",
" ankyrin repeat-containing protein 2 ", " Ankyrin repeat family protein ",
" annexin 1 ", " annexin 7 ", " APS reductase 3 ", " Arginase/deacetylase superfamily protein ",
" aspartate aminotransferase 1 ", " aspartate aminotransferase 2 ",
" aspartate aminotransferase 3 ", " aspartate aminotransferase 5 ",
" ATP-dependent caseinolytic (Clp) protease/crotonase family protein ",
" ATP citrate lyase (ACL) family protein ", " ATP phosphoribosyl transferase 1 ",
" ATP phosphoribosyl transferase 2 ", " ATP sulfurylase 1 ",
" ATP synthase alpha/beta family protein ", " ATP synthase protein I -related ",
" ATP synthase subunit alpha ", " ATP synthase subunit beta ",
" ATPase, F0 complex, subunit B/B', bacterial/chloroplast ",
" ATPase, F1 complex, alpha subunit protein ", " ATPase, F1 complex, gamma subunit protein ",
" ATPase, V0/A0 complex, subunit C/D ", " ATPase, V1 complex, subunit B protein ",
" basic transcription factor 3 ", " beta-1,3-glucanase_putative ",
" Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein ",
" binding to TOMV RNA 1L (long form) ", " branched-chain amino acid aminotransferase 5 / branched-chain amino acid transaminase 5 (BCAT5) ",
" branched-chain aminotransferase 3 ", " branched-chain aminotransferase4 ",
" calcium sensing receptor ", " carbonic anhydrase 1 ", " carbonic anhydrase 2 ",
" catalase 2 ", " chaperonin-60alpha ", " chaperonin 60 beta ",
" chlorophyll A/B binding protein 3 ", " chloroplast heat shock protein 70-1 ",
" chloroplast heat shock protein 70-2 ", " chloroplast RNA binding ",
" chloroplast stem-loop binding protein of 41 kDa ", " chloroplastic NIFS-like cysteine desulfurase ",
" chorismate synthase, putative / 5-enolpyruvylshikimate-3-phosphate phospholyase, putative ",
" cinnamyl alcohol dehydrogenase 9 ", " Citrate synthase family protein ",
" Class I glutamine amidotransferase-like superfamily protein ",
" Clp ATPase ", " Coatomer, alpha subunit ", " Cobalamin-independent synthase family protein ",
" cold, circadian rhythm, and RNA binding 1 ", " cold, circadian rhythm, and rna binding 2 ",
" Coproporphyrinogen III oxidase ", " Cupredoxin superfamily protein ",
" Cyclophilin-like peptidyl-prolyl cis-trans isomerase family protein ",
" cyclophilin 38 ", " cysteine synthase 26 ", " cysteine synthase C1 ",
" Cytosol aminopeptidase family protein ", " cytosolic NADP+-dependent isocitrate dehydrogenase ",
" D-3-phosphoglycerate dehydrogenase ", " D-cysteine desulfhydrase ",
" D-ribulose-5-phosphate-3-epimerase ", " DegP protease 1 ",
" Dehydrin family protein ", " delta tonoplast integral protein ",
" desulfo-glucosinolate sulfotransferase 18 ", " Di-glucose binding protein with Kinesin motor domain ",
" dicarboxylate diiron protein, putative (Crd1) ", " dicarboxylate transport 2.1 ",
" dicarboxylate transporter 1 ", " Dihydrolipoamide succinyltransferase ",
" Disease resistance protein (TIR-NBS-LRR class) family ", " DNA repair ATPase-related ",
" DNAJ heat shock N-terminal domain-containing protein ", " Domain of unknown function (DUF3598) ",
" dual specificity protein phosphatase (DsPTP1) family protein ",
" edited nad9/rpl16 transcript found in intergenic region. From Philippe Giege (CNRS)",
" edited PSBE", " eif4a-2 ", " elicitor-activated gene 3-1 ",
" Enolase ", " epithiospecifier modifier 1 ", " epithiospecifier protein ",
" ethylene-dependent gravitropism-deficient and yellow-green-like 2 ",
" ethylene-forming enzyme ", " Eukaryotic aspartyl protease family protein ",
" eukaryotic initiation factor 4A-III ", " eukaryotic translation initiation factor 2 alpha subunit ",
" Eukaryotic translation initiation factor 2 subunit 1 ", " eukaryotic translation initiation factor 4A1 ",
" FASCICLIN-like arabinogalactan protein 13 precursor ", " FASCICLIN-like arabinoogalactan 9 ",
" ferredoxin-NADP(+)-oxidoreductase 1 ", " ferredoxin-NADP(+)-oxidoreductase 2 ",
" flavanone 3-hydroxylase ", " formate dehydrogenase ", " fructose-bisphosphate aldolase 1 ",
" fructose-bisphosphate aldolase 2 ", " FTSH protease 1 ", " FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cyt",
" GDP-D-mannose 3',5'-epimerase ", " GDSL-like Lipase/Acylhydrolase superfamily protein ",
" germin 3 ", " Glucose-1-phosphate adenylyltransferase family protein ",
" glutamate-1-semialdehyde-2,1-aminomutase ", " glutamate-1-semialdehyde 2,1-aminomutase 2 ",
" glutamate-cysteine ligase ", " glutamate synthase 1 ", " glutamate:glyoxylate aminotransferase ",
" glutamine synthase clone F11 ", " glutamine synthetase 2 ",
" glyceraldehyde-3-phosphate dehydrogenase B subunit ", " glyceraldehyde-3-phosphate dehydrogenase C subunit 1 ",
" glyceraldehyde-3-phosphate dehydrogenase C2 ", " glyceraldehyde-3-phosphate dehydrogenase of plastid 2 ",
" glyceraldehyde 3-phosphate dehydrogenase A subunit ", " glyceraldehyde 3-phosphate dehydrogenase A subunit 2 ",
" Glycine cleavage T-protein family ", " glycine decarboxylase P-protein 1 ",
" GroES-like zinc-binding alcohol dehydrogenase family protein ",
" GroES-like zinc-binding dehydrogenase family protein ", " GTP-binding protein-related ",
" GTP binding ", " GTP binding Elongation factor Tu family protein ",
" heat shock protein 70 (Hsp 70) family protein ", " Heat shock protein 70 (Hsp 70) family protein ",
" heat shock protein 90.1 ", " high chlorophyll fluorescent 109 ",
" high cyclic electron flow 1 ", " histidinol dehydrogenase ",
" Histone superfamily protein ", " homolog of bacterial cytokinesis Z-ring protein FTSZ 1-1 ",
" homoserine kinase ", " HOPW1-1-interacting 1 ", " HSP20-like chaperones superfamily protein ",
" HXXXD-type acyl-transferase family protein ", " Hyaluronan / mRNA binding family ",
" hydroxymethylbilane synthase ", " hydroxyproline-rich glycoprotein family protein ",
" hydroxypyruvate reductase ", " Inositol monophosphatase family protein ",
" Insulinase (Peptidase family M16) protein ", " Involved in response to salt stress. Knockout mutants are hypersensitive to salt stress. ",
" Iron-sulphur cluster biosynthesis family protein ", " isocitrate dehydrogenase subunit 2 ",
" isocitrate dehydrogenase V ", " isocitrate dehydrogenase VI ",
" isopropyl malate isomerase large subunit 1 ", " isopropylmalate dehydrogenase 1 ",
" isopropylmalate dehydrogenase 2 ", " isopropylmalate dehydrogenase 3 ",
" Keratin 1 - Homo sapiens (Human).", " Keratin 10 - Homo sapiens (Human).",
" Keratin 14 (Epidermolysis bullosa simplex, Dowling-Meara, Koebner) - Homo sapiens (Human).",
" Keratin 2a - Homo sapiens (Human).", " Keratin 5 - Homo sapiens (Human).",
" Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9) - Homo sapiens (Human).",
" Keratin, type II cytoskeletal 2 epidermal (Cytokeratin 2e) (K2e) (CK 2e) - Homo sapiens (Human).",
" ketol-acid reductoisomerase ", " lactate/malate dehydrogenase family protein ",
" Lactate/malate dehydrogenase family protein ", " Late embryogenesis abundant protein, group 2 ",
" Leucine-rich repeat (LRR) family protein ", " Leucine-rich repeat protein kinase family protein ",
" light-harvesting chlorophyll-protein complex I subunit A4 ",
" light harvesting complex of photosystem II 5 ", " light harvesting complex photosystem II ",
" light harvesting complex photosystem II subunit 6 ", " lipoxygenase 2 ",
" magnesium chelatase i2 ", " malate dehydrogenase ", " Mannose-binding lectin superfamily protein ",
" MAP kinase 11 ", " metallopeptidase M24 family protein ", " methionine adenosyltransferase 3 ",
" Molecular chaperone Hsp40/DnaJ family protein ", " Molybdenum cofactor sulfurase family protein ",
" monodehydroascorbate reductase 1 ", " monodehydroascorbate reductase 6 ",
" Mov34/MPN/PAD-1 family protein ", " myosin heavy chain-related ",
" NAD(P)-binding Rossmann-fold superfamily protein ", " NAD(P)-linked oxidoreductase superfamily protein ",
" NAD(P)H dehydrogenase C1 ", " NAD(P)H dehydrogenase subunit H ",
" NADH dehydrogenase subunit 7 ", " NagB/RpiA/CoA transferase-like superfamily protein ",
" NDH-dependent cyclic electron flow 1 ", " nitrilase 1 ", " Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase family protein ",
" nitrile specifier protein 3 ", " nitrite reductase 1 ", " non-ATPase subunit 9 ",
" non-intrinsic ABC protein 6 ", " non-photochemical quenching 1 ",
" nuclear factor Y, subunit A2 ", " Nucleic acid-binding proteins superfamily ",
" Nucleic acid-binding, OB-fold-like protein ", " nucleobase-ascorbate transporter 7 ",
" Nucleotidylyl transferase superfamily protein ", " O-acetylserine (thiol) lyase B ",
" O-acetylserine (thiol) lyase isoform C ", " O-methyltransferase 1 ",
" O-methyltransferase family protein ", " ornithine carbamoyltransferase ",
" Oxidoreductase family protein ", " Oxidoreductase, zinc-binding dehydrogenase family protein ",
" oxidoreductases, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor;copper ion",
" oxophytodienoate-reductase 3 ", " P-loop containing nucleoside triphosphate hydrolases superfamily protein ",
" Pectinacetylesterase family protein ", " Peptidase M20/M25/M40 family protein ",
" Peptide chain release factor 1 ", " peroxidase CB ", " Peroxidase superfamily protein ",
" peroxisomal 3-keto-acyl-CoA thiolase 2 ", " peroxisomal 3-ketoacyl-CoA thiolase 3 ",
" peroxisomal NAD-malate dehydrogenase 2 ", " pfkB-like carbohydrate kinase family protein ",
" phenylalanyl-tRNA synthetase class IIc family protein ", " phosphatase-related ",
" phosphoglycerate kinase ", " phosphoglycerate kinase 1 ", " Phosphoglycerate kinase family protein ",
" phospholipid/glycerol acyltransferase family protein ", " phosphoribulokinase ",
" phosphoserine aminotransferase ", " photosynthetic electron transfer A ",
" photosynthetic electron transfer C ", " photosystem I light harvesting complex gene 3 ",
" photosystem I subunit D-2 ", " photosystem I subunit E-2 ",
" photosystem I subunit F ", " Photosystem I, PsaA/PsaB protein ",
" photosystem II reaction center protein A ", " photosystem II reaction center protein B ",
" photosystem II reaction center protein C ", " photosystem II reaction center protein D ",
" photosystem II stability/assembly factor, chloroplast (HCF136) ",
" photosystem II subunit O-2 ", " photosystem II subunit P-1 ",
" photosystem II subunit Q-2 ", " photosystem II subunit QA ",
" phragmoplastin interacting protein 1 ", " Plant stearoyl-acyl-carrier-protein desaturase family protein ",
" plasma-membrane associated cation-binding protein 1 ", " plasma membrane intrinsic protein 1C ",
" plasma membrane intrinsic protein 2A ", " Plastid-lipid associated protein PAP / fibrillin family protein ",
" plastid transcriptionally active 16 ", " plastid transcriptionally active 17 ",
" plastid transcriptionally active 5 ", " prohibitin 2 ", " Proteasome component (PCI) domain protein ",
" Protein kinase superfamily protein ", " Protein of unknown function (DUF677) ",
" Protein of unknown function, DUF642 ", " Protein phosphatase 2C family protein ",
" protochlorophyllide oxidoreductase B ", " protochlorophyllide oxidoreductase C ",
" Pyridine nucleotide-disulphide oxidoreductase family protein ",
" Pyridoxal-dependent decarboxylase family protein ", " Pyridoxal phosphate (PLP)-dependent transferases superfamily protein ",
" pyrimidine 1 ", " pyrimidine d ", " pyruvate dehydrogenase complex E1 alpha subunit ",
" pyruvate dehydrogenase E1 alpha ", " pyruvate dehydrogenase E1 beta ",
" RAB GTPase homolog E1B ", " regulatory particle triple-A ATPase 5A ",
" reversibly glycosylated polypeptide 1 ", " Ribose 5-phosphate isomerase, type A protein ",
" ribosomal protein 1 ", " ribosomal protein L12-A ", " Ribosomal protein L3 family protein ",
" Ribosomal protein L4/L1 family ", " ribosomal protein L5 ",
" Ribosomal protein L6 family protein ", " ribosomal protein S1 ",
" Ribosomal protein S11 family protein ", " ribosomal protein S12A ",
" Ribosomal protein S13/S18 family ", " Ribosomal protein S4 (RPS4A) family protein ",
" Ribosomal protein S5 family protein ", " Ribosomal protein S5/Elongation factor G/III/V family protein ",
" ribosomal protein S6 ", " ribulose-bisphosphate carboxylases ",
" Ribulose bisphosphate carboxylase (small chain) family protein ",
" ribulose bisphosphate carboxylase small chain 1A ", " RNA-binding (RRM/RBD/RNP motifs) family protein ",
" RNA 3'-terminal phosphate cyclase/enolpyruvate transferase, alpha/beta ",
" rotamase CYP 4 ", " rubisco activase ", " S-adenosyl-l-homocysteine (SAH) hydrolase 2 ",
" S-adenosylmethionine synthetase 2 ", " S-adenosylmethionine synthetase family protein ",
" S-domain-1 13 ", " Sec1/munc18-like (SM) proteins superfamily ",
" SecY protein transport family protein ", " sedoheptulose-bisphosphatase ",
" semialdehyde dehydrogenase family protein ", " SERINE CARBOXYPEPTIDASE-LIKE 49 ",
" serine hydroxymethyltransferase 4 ", " serine transhydroxymethyltransferase 1 ",
" SNARE associated Golgi protein family ", " sodium/calcium exchanger family protein / calcium-binding EF hand family protein ",
" solanesyl diphosphate synthase 2 ", " spermidine synthase 1 ",
" spermidine synthase 2 ", " SPFH/Band 7/PHB domain-containing membrane-associated protein family ",
" sterol methyltransferase 2 ", " structural constituent of ribosome ",
" sulfite oxidase ", " sulfotransferase 17 ", " TCP-1/cpn60 chaperonin family protein ",
" tetratricopeptide repeat (TPR)-containing protein ", " Tetratricopeptide repeat (TPR)-like superfamily protein ",
" Thiamin diphosphate-binding fold (THDP-binding) superfamily protein ",
" thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4) ",
" thioredoxin family protein ", " Thioredoxin superfamily protein ",
" thylakoid rhodanese-like ", " thylakoidal ascorbate peroxidase ",
" TRAF-like family protein ", " Transketolase ", " Transketolase family protein ",
" Translation elongation factor EF1B, gamma chain ", " translation initiation factor 3 subunit H1 ",
" triacylglycerol lipase-like 1 ", " trigalactosyldiacylglycerol2 ",
" Tropomyosin-related ", " Trypsin family protein with PDZ domain ",
" Trypsin precursor (EC 3.4.21.4) - Sus scrofa (Pig).", " tryptophan biosynthesis 1 ",
" tryptophan synthase beta-subunit 2 ", " tubulin alpha-3 ",
" tubulin alpha-4 chain ", " tubulin beta chain 2 ", " tubulin beta chain 4 ",
" Tubulin/FtsZ family protein ", " Tyrosine transaminase family protein ",
" UDP-D-apiose/UDP-D-xylose synthase 1 ", " UDP-D-apiose/UDP-D-xylose synthase 2 ",
" UDP-D-glucose/UDP-D-galactose 4-epimerase 1 ", " UDP-glucosyl transferase 74B1 ",
" Uncharacterised conserved protein (UCP030210) ", " unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G16520.1); H",
" unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown",
" unknown protein; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast stroma, chloropl",
" unknown protein; LOCATED IN: chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 ",
" Uroporphyrinogen decarboxylase ", " vacuolar ATP synthase subunit A ",
" vacuolar ATP synthase subunit C (VATC) / V-ATPase C subunit / vacuolar proton pump C subunit (DET3",
" vacuolar ATP synthase subunit H family protein ", " VALINE-TOLERANT 1 "
), class = "factor"), timetE1_1rerun = c(6692.50136510743, 41682.9111356503,
405946.374877924, 4640.34876265179), timetE2_1 = c(14404.8414547167,
40466.9047986558, 638019.540242027, 2397.71968447607), time1 = c(10146.3608040476,
34148.4389867747, 459639.431186888, 10490.8359468475), time2 = c(5951.82142983062,
45616.664822506, 658598.827475944, 3961.96726959746), time3 = c(4048.60493244488,
45729.9856907985, 639686.154465906, 4466.15324797652), time4 = c(59094.47562632,
139889.207295753, 1764684.16499658, 26250.3247678529), time5 = c(27675.589438082,
111309.636536099, 1117027.2897719, 20320.0802981216), time6 = c(26374.057451648,
129781.168773572, 1147967.44539752, 18413.5391459416), time7 = c(43310.0075838041,
96924.6162599508, 1156442.475223, 29061.2479135303), a_mean = c(7774.44229552491,
43374.1166119026, 585562.72426545, 3866.54724117546), b_mean = c(39113.5325249635,
119476.157216344, 1296530.34384725, 23511.2980313616), c_mean = c(23443.9874102442,
81425.1369141232, 941046.53405635, 13688.9226362685)), .Names = c("Name",
"time1", "time2", "time3", "time4",
"time5", "time6", "time7", "time8", "time9",
"a_mean", "b_mean", "c_mean"), row.names = c(NA, 4L), class = "data.frame")
求和结果：
data[445,]
Name time1 time2 time3 time4 time5 time6 time7
colSums <NA> 313508423 268165038 251963845 239573376 282568199 249946497 246142814
time8 time9 a_mean b_mean c_mean
colSums 208003454 256074474 246665241 251369858 219252253
我也在 Excell 中计算了它，所以这就是为什么我知道它发生了变化。我检查了 10 次，我很确定结果发生了变化。c_mean 的总和值应该在第一列（time1）。