我正在运行一个名为Primer 3的命令行程序。它接受一个输入文件并将数据返回到标准输出。我正在尝试编写一个 Ruby 脚本来接受该输入,并将这些条目放入哈希中。
返回的结果如下。我想在 '=' 符号上拆分数据,以便有这样的东西:
{:SEQUENCE_ID => "example", :SEQUENCE_TEMPLATE => "GTAGTCAGTAGACNAT..etc", :SEQUENCE_TARGET => "37,21" etc }
我还想将键小写,即:
{:sequence_id => "example", :sequence_template => "GTAGTCAGTAGACNAT..etc", :sequence_target => "37,21" etc }
这是我当前的脚本:
#!/usr/bin/ruby
puts 'Primer 3 hash'
primer3 = {}
while line = gets do
name, height = line.split(/\=/)
primer3[name] = height.to_i
end
puts primer3
它正在返回:
Primer 3 hash
{"SEQUENCE_ID"=>0, "SEQUENCE_TEMPLATE"=>0, "SEQUENCE_TARGET"=>37, "PRIMER_TASK"=>0, "PRIMER_PICK_LEFT_PRIMER"=>1, "PRIMER_PICK_INTERNAL_OLIGO"=>1, "PRIMER_PICK_RIGHT_PRIMER"=>1, "PRIMER_OPT_SIZE"=>18, "PRIMER_MIN_SIZE"=>15, "PRIMER_MAX_SIZE"=>21, "PRIMER_MAX_NS_ACCEPTED"=>1, "PRIMER_PRODUCT_SIZE_RANGE"=>75, "P3_FILE_FLAG"=>1, "SEQUENCE_INTERNAL_EXCLUDED_REGION"=>37, "PRIMER_EXPLAIN_FLAG"=>1, "PRIMER_THERMODYNAMIC_PARAMETERS_PATH"=>0, "PRIMER_LEFT_EXPLAIN"=>0, "PRIMER_RIGHT_EXPLAIN"=>0, "PRIMER_INTERNAL_EXPLAIN"=>0, "PRIMER_PAIR_EXPLAIN"=>0, "PRIMER_LEFT_NUM_RETURNED"=>0, "PRIMER_RIGHT_NUM_RETURNED"=>0, "PRIMER_INTERNAL_NUM_RETURNED"=>0, "PRIMER_PAIR_NUM_RETURNED"=>0, ""=>0}
数据源
SEQUENCE_ID=example
SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21
PRIMER_TASK=pick_detection_primers
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_OPT_SIZE=18
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=21
PRIMER_MAX_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=75-100
P3_FILE_FLAG=1
SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21
PRIMER_EXPLAIN_FLAG=1
PRIMER_THERMODYNAMIC_PARAMETERS_PATH=/usr/local/Cellar/primer3/2.3.4/bin/primer3_config/
PRIMER_LEFT_EXPLAIN=considered 65, too many Ns 17, low tm 48, ok 0
PRIMER_RIGHT_EXPLAIN=considered 228, low tm 159, high tm 12, high hairpin stability 22, ok 35
PRIMER_INTERNAL_EXPLAIN=considered 0, ok 0
PRIMER_PAIR_EXPLAIN=considered 0, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
$ primer3_core < example2 | ruby /Users/sean/Dropbox/bin/rb/read_primer3.rb