You can use some locking mechanism to make sure the database works in a multithreading situation. Since your application is a read intensive one according to what you said, you can consider using a ReaderWriterLock or ReaderWriterLockSlim. (refer to here and here for more details)
If you have only one database, then creating just one instance of the lock is OK; if you have more than one database, each of them can be assigned a lock. Every time you do some read or write, enter the lock (by EnterReadLock() for ReaderWriterLockSlim, or by AcquireReaderLock() for ReaderWriterLock) before you do something, and after you're done exit the lock. Note that you can place the exit of the lock in a finally clause lest you forget to release it.
The strategy above is being used in our production applications. It's not so good as to use a single thread in your case because you have to take performance into account.