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我之前问过与此类似的问题,但以前的解决方案并没有解决我的问题。我一直在思考和测试东西,但没有任何东西能正常工作。

我有一个包含 500 多个序列的 fasta 文件,我需要从该文件中构建一个表,因此我尝试编写一个脚本来完成它,而不是通过复制粘贴手动完成。我正在使用 Biopython 读取文件:seq=SeqIO.parse(handle, "fasta")

从每个序列中,我想知道蛋白质序列所属的物种、蛋白质的名称和 Uniprot ID。当我使用 SeqIO 解析 fasta 文件时,我注意到我可以从中解析的信息不多。

这是我的 fasta 文件的一个子集:

>gi|194757291|ref|XP_001960898.1| GF11270 [Drosophila ananassae] >gi|190622196|gb|EDV37720.1| GF11270 [Drosophila ananassae] MSAARTSQDCDCTAKCRLRQHGNTITAALTKRSISSQNLAAFVYKTCGNFANILDDLGRSAVHMSASTGRYEILEWLLNH GAYINGQDYESGSSPLHRALYYGSIDCAVLLLRYGASMELLDEDTCCPLQAICRKCDVDDFATDSQNDVLVWGSNKNYNL GVGSEQNTNAPQSVDFFRKSNIWIEQVALGAYHSLFLDKKGHLYAVGHGKGGRLGTGGENTLPAPKRVKVSSKLGSEDSI RCISVSRQHSLVLTHRSLVFACGLNSDCQLGVRDAPEHLAQFKEVVALRDKGASDLVRVIACDQHSIAYGSRCVYVWGAN QGQFGISANIASIVVPTLIKLPARTTIRFVEANNAATVIYSEEKMIYLYYAEKTRAIKTPNYEDLKSISVMGGHIKNSAK GSAAALKLLMLTETNVVYLWYENTQQFYRCNFLPIRLPQIKKILYKCNQVMVLSEDGCVYRGKCNQIALPASELQEKSRP NLDIWQNNDQNRTEISREHVIRIELQRVPNIDRAVDISCDEGFSSFAVLQESQGKYFRKPTLPRKEHSFKKLLHDTSDCD AVHDVVFHVDGEKYPAHKYIIYSRAPGLRELVRMYLDKDIYLNFENLTGKMFELVLKHIYTNYWPTEDDIDCIQQSLGPA NPQNRSRTCQMFLPHLEKFQLTELAKYVKSYVQDHQFPLPSARQRLPRLHRSDYPELYDVKIKCEDGQVLQAHKCMLVAR LEYFEMMFMHSWAERSSVTMEGVPAEYMEPVLDYLYSLEAEAFCKQAYLETFLYNMITICDQYFIESLQNLCELLILDKI SIRKCGEMLEFATMYNCKLLLKGCMDFICQNLARVLCYRSIEQCDGETLKCLNDHYRNMFSRVFDYRQITPFSEAIEDEL LLSFIDGLEVDLEYRMDAESKAKQAAKTKQKDLRKLNARHQYEQRAISSMMRSISISESNPAPEVATSPQESARSETNNW SRVIDKKEQKRKQAETALKVNKTLKQETSPEPEMVPIERTPVNEQTPPPLSPETEPSTPLNKSYNLDFSSLTPQSQKLSQ KQRKRLSSESKSWRGNSSALLESPTTPVPVPNAWGVTTTPSSSFNDSYTSPTTGSSSDPTSFANMMRSQAASSSATSKDQ SQNFSKILADERRQRESYERMRNKSLVHTQIEETAIAELREFYNVDNIDDEKITIARKSRPSDINFSTWIRQ
>gi|198456847|ref|XP_001360463.2| GA20796 [Drosophila pseudoobscura pseudoobscura] >gi|198135774|gb|EAL25038.2| GA20796 [Drosophila pseudoobscura pseudoobscura] MSTAKAQEYDCTAKCTCRQHGNSITAALTKRSIDNQNLGAFIFKTCGNFANIIDDLGRSAVHMSASVARYEILEWLLNHG AYINGLDYESGSSPLHRALYYGSIDCAVLLLRYGASLELLDEDTRCPLQAICRKCDEDFTTESQNDVLVWGSNKNYNLGI GNEQNTNAPQAVDFFRKSNIWIEQVALGAYHSLFCDKKGHLYAVGHGKGGRLGIGVENSLPAPKRVKVSSKLNDDSIMCI SVSRQHSLLLTRRSLVFACGINTDHQLGVRDAPENLTQFREVVALRDKGASDLLRVIACDQHSIAYSTKCVYVWGANQGQ FGISRTTDTIMAPTLIKLPARTSIRFVEANNAATVIYTEEKMITLFYGDKTRYIKTPNYEDLKSIAVIGGHLKSSTKGSA AALKLLMLTETNVVFLWYENTQQFYRCNFSPIRLPEIKKILYKCNQVLILSLDGCVYRGKCNQIALPAGILEEKSKPNMD IWHNNDQNRTEISREHVIRIELQRVPNIDRATDIFCDESFSSFAVLQESHMKYFRKPPLPRREHNFKKLYHDTCESDAVH DVVFHVDGERFAAHKFILYSRAPGLRELTRIYLDKDVYLNFENLTGKMFELILKYIYTSYWPTEDDIDCIQESLGPANPR ERSRACEMFIPHLEMFQLVDLARYLQSYVRDNQFPIPSTRQRFNRLHRSDYPELYDVRIVCEDSKVLEAHKCMLVSRLEY FEMMFTHSWAERTTVNMEGVPAEYMEPVLDYLYSLDTEAFCKQNYTETFLYNMVTFCDQYFIESLQNVCESLILDKISIR KCGEMLDFAAMYNCKLLHKGCMDFICHNLARVLCYRSIEQCDEATLKCLNDHYRKMFSNVFDYRQITPFSEAIEDELLLS FVVDCDIDLDYRMDPETKLKAAAKHKQKDLRRQDARHYYEQQAISSMMRSLSVSESASGPEATTGPQESTRSEGKNWSRV VDKKEQKRKLADTALKVNNTLKLEEPPRPELEVIERALMKEQTPPPTSPAEETSTPLSKSYNLDLSSLTPQSQKLSQKQR KRLSSESKSWRSPLVEQEPTTPVAVPNAWGLPPATPSSSSFTDSPATGSISDPTSFANMMRGQAAAATTPTEKGQSFSRI LADERRQRESFERMRNKSLAHTQIEETAIAELREFYNVDNTDDETITIERKSRPTDINFSTWLKH
>gi|355695434|gb|AES00009.1| inhibitor of Bruton agammaglobulinemia tyrosine kinase [Mustela putorius furo] KPGNKLKLNQKKCSFLCDVTMKSVDGKEFTCHKCVLCARLEYFHSMLSSSWIEASTCTALEMPIHSDILKVILDYLYTDE AVVIKESQNVDFVCSVLVVADQLLITRLKGMCEVALTEKLTLKNAAMILEFAAMYNAEQLKLSCLQFIGLNM

有什么方法可以从这些序列中获取蛋白质名称、Uniprot ID 和生物体?例如,我想从 seq.description 中解析基因库 ID,然后使用该 ID 在基因库中搜索,但我认为无法做到,并非所有序列都有基因库 ID。任何建议如何做到这一点?任何帮助将非常感激。

所需输出的示例:

name    organism    uniprot id  family
GF11270 Sophophora  B3MFN0  
GA20796 Sophophora  Q291S4  
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1 回答 1

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你也可以问 biostar: http: //www.biostars.org/

从您的 fasta 标头中提取 ACN。例如:GF11270

并使用 uniprot REST API 检索与此 ACN 关联的记录

http://www.uniprot.org/uniprot/?query=GF11270&sort=score&format=xml

http://www.uniprot.org/uniprot/?query=GF11270&sort=score&format=txt

http://www.uniprot.org/uniprot/?query=GF11270&sort=score&format=tab

于 2013-02-13T23:43:14.600 回答