Your problem is not calling the method from another class. I suppose UserControl1 is your custom user control, and that the "class 2" you mentioned is a userControl1.
The code would work as it is, but you are calling it on the wrong instance of that control.
In your btn_login_Click method, you are generating a totally new instance of UserControl1. You are of course allowed to do that, which is why Visual Studio would never mark it as an error, but uc1 will not be the control that actually sits in your form.
Let's say in your form you have named the control "cbxOptions". Then in the button click event, you have to write
cbxOptions.fill_cbb();
instead, if that combobox is also of type UserControl1. Then it should work just fine.
Warning, car analogy: It's like when you want a new paint job on your car. Then you buy a new car of the same model and take that to the paint shop, get it painted, then bring it to the junkyard and get it crushed. Your old car will still have the same old color of course.