我必须在基因组序列的FASTA 文件wTTTAYRTTTW中搜索模式，其中W= Aor T, Y= Cor T, R= Aor 。应该允许一些不匹配，即完全匹配字符串及其位置。我的做法是：R
#!user/bin/perl -w
use strict;
my @name = qw( NC_004314.2_10 );
for (my $i = 0 ; $i< scalar(@name);$i++)
{
my $fname = $name[$i];
print "$fname\n";
my $read_pat= "WTTTAYRTTTW";
#print"\nPlease enter how many mismatch is allowd : ";
my $m =<STDIN>;
chomp $m;
unless(open(fh1, "$fname")){
print "Cannot open file \"$fname\"\n\n";
exit;
}
my @fh=<fh1>;
close fh1;
if ($fh[0] !~ /^>/)
{
print "not fasta file\n";
exit;
}
my $seq='';
foreach my $line(@fh)
{
if($line =~ /^>/)
{
next;
}
else
{
$seq=$seq.$line;
}
}
sub trans_pat
{
my $pat=shift;
$pat=~s/R/\[CG\]/g;
$pat=~s/W/\[AT\]/g;
$pat=~s/Y/\[AG\]/g;
return $pat;
}
open(FH1,">$fname.csv");
sub find_pat
{
my ($pat,$seq) = (@_);
#print FH1 "Looking for pattern $pat\n";
}
find_pat (trans_pat($read_pat),$seq);
# Allowing for a single mismatch
my $pat=trans_pat($read_pat);
print FH1 "Looking for pattern $pat\n";
while ($seq=~m/(?=$pat)/g)
{
print FH1"match at\t$-[0]\t$&\n"
}
foreach my $i (1..(length $read_pat)-($m-1))
{
my $mis_pat = $read_pat;
substr($mis_pat,$i-1,$m)=".{$m}";
my $pat1=trans_pat($mis_pat);
print FH1 "Looking for pattern $pat1\n";
while ($seq=~m/(=?$pat1)/g)
{
print FH1 "match at\t$-[0]\t$&\n";
}
#print FH1"$& \n";
}
close FH1;
这段代码发现的结果对于FASTA文件中给定序列NC_004314.2的精确匹配是错误的，总匹配数应该是829。任何人都可以纠正这个代码吗？